Asymmetrical Flow Field-Flow Fractionation

The universal method among the FFF-techniques

 

Field-flow fractionation (FFF) comprises several flexible elution techniques for simultaneous separation and measurement. Separation takes place in a thin, open channel through which samples are transported by a laminar flow of liquid. A field, applied perpendicularly to the channel flow, partitions the various components into regions of different flow velocities.

Asymmetrical flow FFF (AFFFF) is a type of flow FFF using only one permeable wall which serves as accumulation wall. Experiments show that the asymmetrical design separates faster and more efficient than the symmetrical channel.

AFFFF is certainly one of the most universal FFF techniques. It features a very broad (10⁵⁾ separation range from 10^-3 to 10² ?m . And its application range covers both simple and complex macromaterials of biological, pharmaceutical, industrial and environmental relevance. 

  

 

Applications:

Characterization of macromolecular materials is one of the most important and demanding tasks for scientists worldwide. Research work in these fields concerns materials from:

 

. Medicine

. Biology

. Pharmacology

. Agriculture

. Polymer Science

 and others

Advantages of our AFFFF system:

• broad range of sample material
• broad size range (Mw 1.000(g/mol) - 50 ?m diameter)
• no shear forces
• no influence of adsorption effects
• easy adjustment of separation conditions to specific problems
• separation by hydrodynamic size of the sample

Some typical sample materials for AFFFF analysis: 

SAMPLE MATERIAL	MOL. MASS (G/MOL)
globular proteins	13.000 - 670.000
viruses
ribosomes	10 - 30 nm (diam.)
nucleic acids	4.000.000 - 7.300.000
dextran	40.000 - 500.000
modified celluloses	10.000 - 1.000.000
polyvinyl amin	up to 300.000
polyacryl acid	up to 300.000
polyethylene oxide	18.000 - 880.000
polyvinyl pyrrolidone	up to 400.000
polyethylene imine	up to 4.000.000
polystyrene sulfonate	5.000 - 1.000.000
ps-latex beads	0.001 - 10 ?m (diam.)
particles (silica)	0.001 - 20 ?m (diam.)
polystyrene (THF)	2000-100.000.000
Starch
Polysaccharide
Microgele
Liposome

Our AFFFF-system solves difficult problems the easy way - in research and production environments as well.

 

ConSenxus GmbH * Binger Strasse 17 * 55437 Ober-Hilbersheim * Germany * Tel:+49-672894056 * Fax:+49-672894058 * info@consenxus.de